Stability of plasma adrenocorticotrophic hormone (ACTH): influence of hemolysis, rapid chilling, time, and the addition of a maleimide.

@article{Livesey2010StabilityOP,
  title={Stability of plasma adrenocorticotrophic hormone (ACTH): influence of hemolysis, rapid chilling, time, and the addition of a maleimide.},
  author={John Hamilton Livesey and Barbara Dolamore},
  journal={Clinical biochemistry},
  year={2010},
  volume={43 18},
  pages={1478-80}
}
OBJECTIVES The aim of this study was to examine the effects of hemolysis, rapid chilling, time, and the addition of a maleimide on the stability of human plasma ACTH measurements. DESIGN AND METHODS Partially hemolyzed EDTA blood (n=10), initially at 37°C, was centrifuged at 4°C either immediately or after rapid chilling in ice/water. Plasma ACTH was then measured either immediately, or after 1h at 22°C with or without the addition of 2mM N-phenyl maleimide (NPM). RESULTS For 0.2% hemolysis… CONTINUE READING