Stability of four steroids in lyophilised human serum.


The stability of a preparation of lyophilised serum and its suitability for use as a reference material for routine assays of cortisol, oestradiol, and progesterone have been studied in four laboratories with a variety of assay systems. Cortisol and oestradiol were also measured by gas chromatography-mass spectrometry. The lyophilised serum was suitable for use in all routine and reference assay methods examined, with between-method variability no greater than that for frozen serum pools. The concentrations of cortisol, oestradiol, and progesterone were predicted by accelerated degradation studies to decline by 0.01% per annum if the preparations were to be stored at --20 degrees C. The testosterone content of the preparation, determined in one laboratory, provided no evidence for degradation. The preparation can be shipped for use at ambient temperature without deleterious effect.

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@article{Das1983StabilityOF, title={Stability of four steroids in lyophilised human serum.}, author={Rose E. Gaines Das and Derek Calam and Francine L Mitchell and F P Woodford and Marion L Cawood and Simon J. Gaskell and Barry Joyce and Mike McMillan and M. Wheeler}, journal={Annals of clinical biochemistry}, year={1983}, volume={20 (Pt 6)}, pages={364-8} }