Stability of four steroids in lyophilised human serum.

Abstract

The stability of a preparation of lyophilised serum and its suitability for use as a reference material for routine assays of cortisol, oestradiol, and progesterone have been studied in four laboratories with a variety of assay systems. Cortisol and oestradiol were also measured by gas chromatography-mass spectrometry. The lyophilised serum was suitable for use in all routine and reference assay methods examined, with between-method variability no greater than that for frozen serum pools. The concentrations of cortisol, oestradiol, and progesterone were predicted by accelerated degradation studies to decline by 0.01% per annum if the preparations were to be stored at --20 degrees C. The testosterone content of the preparation, determined in one laboratory, provided no evidence for degradation. The preparation can be shipped for use at ambient temperature without deleterious effect.

Cite this paper

@article{Das1983StabilityOF, title={Stability of four steroids in lyophilised human serum.}, author={Rose E. Gaines Das and Derek Calam and Francine L Mitchell and F P Woodford and Marion L Cawood and Simon J. Gaskell and Barry Joyce and Mike McMillan and M. Wheeler}, journal={Annals of clinical biochemistry}, year={1983}, volume={20 (Pt 6)}, pages={364-8} }