Stability of commonly used thiols and of human creatine kinase isoenzymes during storage at various temperature in various media.

@article{Nealon1977StabilityOC,
  title={Stability of commonly used thiols and of human creatine kinase isoenzymes during storage at various temperature in various media.},
  author={Daniel A. Nealon and Arthur R. Henderson},
  journal={Clinical chemistry},
  year={1977},
  volume={23 5},
  pages={816-29}
}
We assessed the stability--at 20, 4 --20, and --80 degrees C--of 2-mercaptoethanol, dithioerythritol, and dithiothreitol, and of semi-purified creatine kinase isoenzymes of human origin in the presence and absence of the three compounds. The isoenzymes plus the sulfhydryl compounds were also assessed an aqueous buffer, in heat-inactivated pooled sera, or in fresh pooled sera with low endogenous total creatine kinase. We also examined the influence of thawing conditions from--20 or--80 degrees C… CONTINUE READING

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