The phenomenology of Standard Model and New Physics at hadron colliders depends critically on results from global QCD analysis for parton distribution functions (PDFs). The accuracy of the standard next-to-leading-order (NLO) global analysis, nominally a few percent, is generally well matched to the expected experimental precision. However, serious questions have been raised recently about the stability of the NLO analysis with respect to certain inputs, including the choice of kinematic cuts on the data sets and the parametrization of the gluon distribution. In this paper, we investigate this stability issue systematically within the CTEQ framework. We find that both the PDFs and their physical predictions are stable, well within the few percent level. Further, we have applied the Lagrange Multiplier method to explore the stability of the predicted cross sections for W production at the Tevatron and the LHC, since W production is often proposed as a standard candle for those colliders. We find the NLO predictions on σ W to be stable well within their previously-estimated uncertainty ranges.