Stability of Integrated Plasmids in the Chromosome of Lactococcus lactis.

@article{Leenhouts1990StabilityOI,
  title={Stability of Integrated Plasmids in the Chromosome of Lactococcus lactis.},
  author={Kees J Leenhouts and Jan Kok and Gerard A. Venema},
  journal={Applied and environmental microbiology},
  year={1990},
  volume={56 9},
  pages={2726-35}
}
Derivatives of plasmids pBR322, pUB110, pSC101, and pTB19, all containing an identical fragment of lactococcal chromosomal DNA, were integrated via a Campbell-like mechanism into the same chromosomal site of Lactococcus lactis MG1363, and the transformants were analyzed for the stability of the integrated plasmids. In all cases the erythromycin resistance gene of pE194 was used as a selectable marker. Transformants obtained by integration of the pBR322 derivatives contained a head-to-tail… CONTINUE READING

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