Stability, activity and structure of adenylate kinase mutants.

  title={Stability, activity and structure of adenylate kinase mutants.},
  author={Peter Sp{\"u}rgin and U Abele and Georg E. Schulz},
  journal={European journal of biochemistry},
  volume={231 2},
Sequence/structure relationships have been explored by site-directed mutagenesis using a structurally known adenylate kinase. In particular the effects of helix capping and nonpolar core expansion on thermodynamic stability have been analyzed. Six point mutations were produced and characterized by SDS/PAGE, native PAGE, isoelectric focussing, electrophoretic titration, enzyme kinetics, and X-ray structure analysis. Heat-denaturation experiments yielded melting temperatures Tm and melting… CONTINUE READING