Stabilisation of a (betaalpha)8-barrel protein designed from identical half barrels.

  title={Stabilisation of a (betaalpha)8-barrel protein designed from identical half barrels.},
  author={T. Seitz and Marco Bocola and J{\"o}rg Claren and Reinhard Sterner},
  journal={Journal of molecular biology},
  volume={372 1},
It has been suggested that the common (betaalpha)(8)-barrel enzyme fold has evolved by the duplication and fusion of identical (betaalpha)(4)-half barrels, followed by the optimisation of their interface. In our attempts to reconstruct these events in vitro we have previously linked in tandem two copies of the C-terminal half barrel HisF-C of imidazole glycerol phosphate synthase from Thermotoga maritima and subsequently reconstituted in the fusion construct HisF-CC a salt bridge cluster… CONTINUE READING