SsrA-mediated tagging in Bacillus subtilis.

  title={SsrA-mediated tagging in Bacillus subtilis.},
  author={Thomas Wiegert and Wolfgang Schumann},
  journal={Journal of bacteriology},
  volume={183 13},
A general mechanism in bacteria to rescue stalled ribosomes involves a stable RNA encoded by the ssrA gene. This RNA, termed tmRNA, encodes a proteolytic peptide tag which is cotranslationally added to truncated polypeptides, thereby targeting them for rapid proteolysis. To study this ssrA-mediated mechanism in Bacillus subtilis, a bipartite detection system was constructed that was composed of the HrcA transcriptional repressor and the bgaB reporter gene coding for a heat-stable beta… CONTINUE READING
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The tmRNA website. Nucleic Acids Res. 28:168

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