SspB delivery of substrates for ClpXP proteolysis probed by the design of improved degradation tags.

@article{Hersch2004SspBDO,
  title={SspB delivery of substrates for ClpXP proteolysis probed by the design of improved degradation tags.},
  author={Greg L. Hersch and Tania A. Baker and Robert T. Sauer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2004},
  volume={101 33},
  pages={12136-41}
}
The ssrA-degradation tag sequence contains contiguous binding sites for the SspB adaptor and the ClpX component of the ClpXP protease. Although SspB normally enhances ClpXP degradation of ssrA-tagged substrates, it inhibits proteolysis under conditions that prevent tethering to ClpX. By increasing the spacing between the protease and adaptor-binding determinants in the ssrA tag, substrates were obtained that displayed improved SspB-mediated binding to and degradation by ClpXP. These extended… CONTINUE READING