Src triggers circular ruffling and macropinocytosis at the apical surface of polarized MDCK cells.

@article{Mettlen2006SrcTC,
  title={Src triggers circular ruffling and macropinocytosis at the apical surface of polarized MDCK cells.},
  author={Marcel Mettlen and Anna Platek and Patrick Van der Smissen and Sarah M. Carpentier and Mustapha Amyere and Letizia Lanzetti and Philippe de Diesbach and Donatienne Tyteca and Pierre J. Courtoy},
  journal={Traffic},
  year={2006},
  volume={7 5},
  pages={589-603}
}
We addressed the role of Src on cortical actin dynamics and polarized endocytosis in MDCK cells harboring a thermosensitive v-src mutant. Shifting monolayers established at 40 degrees C (non-permissive temperature) to 34 degrees C (permissive temperature) rapidly reactivated v-Src kinase, but tight junctions and cell polarity resisted for >6 h. At this interval, activated v-src was recruited on apical vesicles, induced cortactin-associated apical circular ruffles productive of macropinosomes… CONTINUE READING