Src kinase and PI 3-kinase as a transduction pathway in ceramide-induced contraction of colonic smooth muscle.

@article{Ibitayo1998SrcKA,
  title={Src kinase and PI 3-kinase as a transduction pathway in ceramide-induced contraction of colonic smooth muscle.},
  author={Adenike I Ibitayo and Y Tsunoda and F Nozu and Chung Owyang and Khalil N. Bitar},
  journal={The American journal of physiology},
  year={1998},
  volume={275 4 Pt 1},
  pages={G705-11}
}
Ceramide mediates sustained contraction of smooth muscle cells. C2 ceramide induced a rapid increase in Src kinase activity within 15 s, peaked at 1 min, and was sustained up to 8 min. Contraction and Src kinase activity were inhibited in cells incubated in Ca2+-free medium containing 2 mM EGTA and in cells preincubated with herbimycin A, a Src kinase inhibitor. Immunoblotting using a phosphospecific anti-Src (416Y) antibody showed a ceramide-induced increase in pp60(src) tyrosine… CONTINUE READING