Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

@article{Hsu2001Spz1AN,
  title={Spz1, a novel bHLH-Zip protein, is specifically expressed in testis},
  author={Shih-Hsien Hsu and Huey-Wen Shyu and Hsiu-Mei Hsieh-Li and Hung Li},
  journal={Mechanisms of Development},
  year={2001},
  volume={100},
  pages={177-187}
}
We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of… CONTINUE READING