Spot overlapping in two-dimensional polyacrylamide gel electrophoresis separations: a statistical study of complex protein maps.

A statistical approach able to extract the information contained in a two-dimenisional polyacrylamide gel electrophoresis (2-D PAGE) separation is here reported. The method is based on the quantitative theory of peak overlapping, a procedure previously developed by the authors and here extended to 2-D separations. The whole map is divided into many strips… CONTINUE READING