Spot-immunodetection of conserved determinants in eukaryotic RNA polymerases. Study with antibodies to yeast RNA polymerases subunits.

@article{Huet1982SpotimmunodetectionOC,
  title={Spot-immunodetection of conserved determinants in eukaryotic RNA polymerases. Study with antibodies to yeast RNA polymerases subunits.},
  author={Janine Huet and A. Sentenac and Pierre Fromageot},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 5},
  pages={
          2613-8
        }
}
We have extended our immunological studies of yeast nuclear RNA polymerases (Buhler, J. M., Huet, J., Davies, K., Sentenac, A., and Fromageot, P. (1980) J. BioL Chem 255,9949-9954) and explored the immunological relationships of form B RNA polymerase from yeast, plant, insect, crustacea, and mammal. The three forms of RNA polymerases from Saccharomyces cerevisiae were spotted on nitrocellulose filters and then probed with specific antibodies directed against each polypeptide component of yeast… CONTINUE READING

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