Spot blot: a hybridization assay for specific DNA sequences in multiple samples.

@article{Cunningham1983SpotBA,
  title={Spot blot: a hybridization assay for specific DNA sequences in multiple samples.},
  author={Mitchell Cunningham},
  journal={Analytical biochemistry},
  year={1983},
  volume={128 2},
  pages={
          415-21
        }
}
Small aliquots of DNA-containing samples from gradient or column fractions are spotted onto agarose plates containing ethidium bromide, and the DNA is visualized qualitatively. After it is overlaid with a thin agarose cover, the DNA is denatured and transferred to nitrocellulose filters for hybridization. This method, called the "spot-blot assay," is particularly convenient when dealing with large numbers of samples.