Sporulation genes in members of the low G+C Gram-type-positive phylogenetic branch (Firmicutes)

@article{Onyenwoke2004SporulationGI,
  title={Sporulation genes in members of the low G+C Gram-type-positive phylogenetic branch (Firmicutes)},
  author={Rob Uche Onyenwoke and Julie A. Brill and Kamyar Farahi and Juergen Wiegel},
  journal={Archives of Microbiology},
  year={2004},
  volume={182},
  pages={182-192}
}
Endospore formation is a specific property found within bacteria belonging to the Gram-type-positive low G+C mol% branch (Firmicutes) of a phylogenetic tree based on 16S rRNA genes. Within the Gram-type-positive bacteria, endospore-formers and species without observed spore formation are widely intermingled. In the present study, a previously reported experimental method (PCR and Southern hybridization assays) and analysis of genome sequences from 52 bacteria and archaea representing… 

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References

SHOWING 1-10 OF 36 REFERENCES

Sigma factor and sporulation genes in Clostridium.

TLDR
The picture emerges that a fundamental similarity exists at the genetic level between the regulation of various cellular responses, in particular sporulation, in the genera Bacillus and Clostridium.

Phylogenetic status of Anaerobacter polyendosporus, an anaerobic, polysporogenic bacterium.

TLDR
It was shown that this polysporogenic bacterium belongs to the Clostridium cluster I, subcluster A and possesses such common phenotypic features as a Gram-positive cell wall structure, anaerobiosis, derivation of energy from carbohydrate fermentation yielding butyric acid among other organic acids and the capacity for endogenous spore-formation.

Phylogenetic evidence that the gram-negative nonsporulating bacterium Tissierella (Bacteroides) praeacuta is a member of the Clostridium subphylum of the gram-positive bacteria and description of Tissierella creatinini sp. nov.

TLDR
Although T. praeacuta is gram negative and does not produce endospores, 16S rRNA sequence data showed that it is closely related genealogically to Clostridium hastiforme, and a second species of Tissierella, TISSierella creatinini sp.

Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)

TLDR
The 8,667,507 base pair linear chromosome of Streptomyces coelicolor is reported, containing the largest number of genes so far discovered in a bacterium.

Phylogenetic analysis of Metabacterium polyspora: clues to the evolutionary origin of daughter cell production in Epulopiscium species, the largest bacteria

TLDR
A 16S rRNA-based phylogenetic analysis of Metabacterium polyspora, a multiple-endospore-forming, uncultivated inhabitant of guinea pig cecum, showed that the process of sporulation was modified in a predecessor of Epulopiscium spp.

Phylogenetic, amino acid content and indel analyses of the beta subunit of DNA-dependent RNA polymerase of gram-positive and gram-negative bacteria.

TLDR
The rpoB gene, encoding the beta subunit of DNA-dependent RNA polymerase, is sequenced from a selection of gram-positive and gram-negative bacteria to support the division of the gram- positive organisms into two clades based on DNA G+C content.

A Gene Odyssey: Exploring the Genomes of Endospore-Forming Bacteria

TLDR
This chapter provides hints about how much of the sporulation process and its regulation is conserved and indicates that all the genes involved in the B. subtilis phosphorelay are present in B. anthracis and B. stearothermophilus, with the possible exception of spoOE.

The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations.

TLDR
The 16S rRNA gene sequences were determined by PCR direct sequencing and were compared with more than 80 previously determined clostridial sequences and the previously published sequences of representative species of other low- G + C-content gram-positive genera, thereby providing an almost complete picture of the genealogical interrelationships of theClostridia.

Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum

TLDR
The data presented here provide strong evidence that at least some of the complex regulation features of sporulation in B. acetobutylicum and possibly Clostridium spp.