Spontaneous transgenesis of human B lymphocytes.

@article{Filaci2004SpontaneousTO,
  title={Spontaneous transgenesis of human B lymphocytes.},
  author={Gilberto Filaci and Mara Gerloni and Marta Rizzi and Paola Castiglioni and H-D Chang and Matthew C. Wheeler and R Fiocca and Maurizio Zanetti},
  journal={Gene therapy},
  year={2004},
  volume={11 1},
  pages={42-51}
}
DNA can cross the cell membrane by natural means, but the functional relevance of this phenomenon has not been fully elucidated. Here, we analyzed spontaneous transgenesis of human B cells using plasmid DNA coding for a functional immunoglobulin (Ig) heavy chain gene under the control of a B-cell-specific promoter. Using polymerase chain reaction (PCR), reverse transcriptase-PCR, and flow cytometry in combination, spontaneous transgenesis was documented in Burkitt's lymphoma cell lines, Epstein… CONTINUE READING

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