Spontaneous recombinase activity of Cre–ERT2 in vivo

@article{Kristianto2017SpontaneousRA,
  title={Spontaneous recombinase activity of Cre–ERT2 in vivo},
  author={Jasmin Kristianto and Michael G. Johnson and Ryley K Zastrow and Abigail B. Radcliff and Robert D. Blank},
  journal={Transgenic Research},
  year={2017},
  volume={26},
  pages={411-417}
}
Inducible Cre–ERT recombinase technology is widely used for gene targeting studies. [] Key Method We utilized both a reporter mouse (B6.129 (Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J) and endothelin converting enzyme-1 floxed transgenic mouse line to evaluate Cre–ERT2 activity. We observed spontaneous Cre activity in both settings. Unintended Cre activity is a confounding factor that has a potentially large impact on data interpretation. Thus, it is important to consider background Cre activity in…
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