Spontaneous reactivation of Shiga toxins in Escherichia coli O157:H7 cells caused by transposon excision.

@article{Kusumoto2001SpontaneousRO,
  title={Spontaneous reactivation of Shiga toxins in Escherichia coli O157:H7 cells caused by transposon excision.},
  author={Masahiro Kusumoto and Tadayuki Okitsu and Yoshiaki Nishiya and Rieko Suzuki and Shiro Yamai and Yoshiaki Kawamura},
  journal={Journal of bioscience and bioengineering},
  year={2001},
  volume={92 2},
  pages={114-20}
}
IS1203v is an insertion sequence which has been found in inactivated Shiga toxin 2 genes (stx2) of Escherichia coli O157:H7. Using PCR amplification, we detected the wild-type stx2 genes in colonies of E. coli O157:H7 which possessed stx2 genes inactivated by insertion of IS1203v. This suggests that IS1203v is excised from the inactivated stx2 genes in E. coli O157:H7. We isolated the cells possessing the wild-type stx2 genes, and confirmed Stx2 productivities by reversed passive latex… CONTINUE READING