Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. IX. The quantitation of natural killer cell activity

@article{Pross2004SpontaneousHL,
  title={Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. IX. The quantitation of natural killer cell activity},
  author={Hugh F. Pross and Malcolm G. Baines and Peter Rubin and Peter C. Shragge and Michael S. Patterson},
  journal={Journal of Clinical Immunology},
  year={2004},
  volume={1},
  pages={51-63}
}
On analysis ofin vitro assays of human natural killer (NK) cell function the inadequacy of commonly used methods of expressing lytic activity was apparent. A comparison was made of the data obtained using modifications of two equations—the simple exponential fit and the von Krogh equations. Both of these equations were found to satisfy the following essential criteria for use in these assays. First, the majority of the results obtained in the chromium-release assay could be used in data… 
View on Springer
ncbi.nlm.nih.gov
224 Citations
Highly Influential Citations
2
Background Citations
23
Methods Citations
50

Figures and Tables from this paper

224 Citations

Citation Type

Citation Type

Kinetic analysis of cytotoxic lymphocyte-target cell interaction as quantified by dual parameter flow cytometry.
TLDR
The concordance of theoretical curves with experimental data proved that the described model can be considered as a useful tool for the evaluation of kinetic and dynamic characterization of conjugate formation between leukemic targets and LAK effectors.
Studies of human natural killer cells. III. Neutropenia associated with unusual characteristics of antibody-dependent and natural killer cell-mediated cytotoxicity
TLDR
A 52-year-old Caucasian man with chronic neutropenia and recurrent infections was found to have an increased proportion of peripheral T lymphocytes having Fc receptors for IgG (T(γ), which suggested that ADCC and NK were mediated by the same cell type, albeit acting by different mechanisms.
Role of proliferation in LAK cell development
TLDR
The data suggests that not allLAK activity is dependent on proliferation, and that high levels of proliferation in the presence of IL-2 do not necessarily lead to LAK activity.
Regulation of human natural killer (NK) cell function: Induction of killing of an NK-resistant renal carcinoma cell line
TLDR
The present studies demonstrate that the cytotoxic functions defined by the ability to lyse these two targets differ in response to a variety of immunoregulatory stimuli.
A fluorescence-based assay for quantitation of lymphokine-activated killer cell activity.
Improving methods of assessing natural killer cell cytotoxicity
TLDR
A new model for interpreting NK cytotoxic data is presented, introducing maximal target cell lysis and the slope of the cytolytic curve as parameters that attempt to make full use of the information and the statistical power in NK cell cytotoxicity data.
Enhancement of the antibody-dependent cellular cytotoxicity of human peripheral blood lymphocytes with interleukin-2 and interferon α
TLDR
It is shown that activation of the NK cells with a combination of rIL-2and rIFNα induced significantly higher levels of ADCC than either cytokine alone, which may enhance the therapeutic efficacy of mAb by cytokines that are able to enhance the ADCC of human CD3−, CD56+, CD16+ natural killer (NK) cells.
NK-mediated reduction of malignancy in human melanoma cells treated with theophylline
TLDR
The data suggest that theophylline can act preferentially on certain tumor cell subpopulations to enhance their NK-sensitive phenotype and thereby inhibit their capacity to form tumors and to metastasize in nude mice.
Natural Killer Cell Cytotoxicity: A Methods Analysis of Versus Flow Cytometry Chromium Release
TLDR
The authors found no compelling reason to adopt NK cell cytotoxicity by flow cytometry over 51 Cr release, and intra-assay variability between methods differed only at the lowest effector-target ratios evaluated.
Natural killer cell activity against a variety of target cell lines in normal persons: NK-target sensitivity and effect of age and sex on NK levels.
TLDR
It was shown that no sex or age difference existed with respect to NK function, except for a slight but significant decreased in NK activity of young female to K562 target cells, which is considered to remain stable and polyspecific in the results.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 56 REFERENCES
Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. IX. The quantitation of natural killer cell activity.
Kinetic analysis of target cell destruction by effector T cells. I. Delineation of parameters related to the frequency and lytic efficiency of killer cells.
TLDR
The Kh value for a number of lymphoid cell populations from identically immunized C57BL/6 mice was found to vary considerably, thereby providing direct evidence that effector populations have different lytic efficiencies.
Spontaneous cytotoxicity of cultured human cell lines mediated by normal peripheral blood lymphocytes. III. Kinetic parameters.
TLDR
It was shown that SCMC approaches simple Michaelis-Menten enzyme kinetics, allowing determination of a Michaelis constant, Km, and maximal velocity, Vmax, for the interaction between a given effector and target cell.
Cytotoxic activity of human lymphocytes: quanitative analysis of T cell and K cell cytotoxicity, revealing enzyme-like kinetics.
TLDR
Experimental conditions are proposed for optimal measurement of cellular cytotoxicity using a simple rate equation and it was shown that in the ADL a K cell can recycle, i.e., kill more than one target cell.
Characterization of cells that suppress the cytotoxic activity of T lymphocytes. I. Quantitative measurement of inhibitor cells.
TLDR
The ability to quantitate CTL and inhibitor activities from titration curves provides a technique for studying the identity and mechanism of suppressor cells acting at the effector stage of cell-mediated immunity.
Spontaneous human lymphocyte‐mediated cytotoxicity againts tumour target cells. I. The effect of malignant disease
TLDR
Lymphocytes from all normal donors tested showed significant activity in the SLMC assay, with some variation from day to day, and lymphocytes from many patients with malignant disease had decreased SLM activity, and this decrease was highly significant in patients with treated or untreated metastatic disease, or untreated CLL.
Spontaneous human lymphocyte‐mediated cytotoxicity against tumor target cells. V. The role of serum‐derived heterologous membrane antigens
TLDR
Persistence of susceptibility to cytotoxicity after 10 months of culture in 5% human serum, partial sensitivity to pronase and trypsin, and resistance to neuraminidase, all indicated that the antigen recognized by the effector cells in this assay is distinct from the heterologous membrane antigen described by Irie et al.
Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. VII. The effect of immunodeficiency disease.
Natural Killer Cells: Characteristics and Regulation of Activity
TLDR
The views on the characteristics of NK cells are summarized and a few issues of current interest in the laboratory are focused on.
Cytotoxic lymphocytes from normal donors. A functional marker of human non-T lymphocytes.
TLDR
SLMC against xenogeneic targets has been evaluated as a potential marker of lumphocytotoxic function and it has been demonstrated that the cytotoxic activity lies in the non-T-lymphocyte fraction, and is probably caused by the complement receptor-bearing lymphocyte.
...
1
2
3
4
5
...