Spontaneous formation of highly functional three-dimensional multilayer from human hepatoma Hep G2 cells cultured on an oxygen-permeable polydimethylsiloxane membrane.

@article{Evenou2010SpontaneousFO,
  title={Spontaneous formation of highly functional three-dimensional multilayer from human hepatoma Hep G2 cells cultured on an oxygen-permeable polydimethylsiloxane membrane.},
  author={Fanny Evenou and Teruo Fujii and Yasuyuki Sakai},
  journal={Tissue engineering. Part C, Methods},
  year={2010},
  volume={16 2},
  pages={311-8}
}
When considering high-density liver cell cultures, adequate delivery of oxygen to the cells appears particularly crucial since it is one of the most limiting parameters of hepatocytes' functions. Here we report on the effects of direct oxygenation through a gas-permeable polydimethylsiloxane membrane on liver-derived cell culture. We used highly proliferative human hepatoma Hep G2 cells to assess the growth-related limitation of such direct oxygen supply. As a consequence of the greater oxygen… CONTINUE READING

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