Spontaneous establishment and characterization of mouse keratinocyte cell lines in serum-free medium

@article{Kaighn1988SpontaneousEA,
  title={Spontaneous establishment and characterization of mouse keratinocyte cell lines in serum-free medium},
  author={M. Edward Kaighn and Richard F. Camalier and Federico Bertolero and Umberto Saffiotti},
  journal={In Vitro Cellular & Developmental Biology},
  year={1988},
  volume={24},
  pages={845-854}
}
Mouse keratinocytes cultures readily develop into established cell lines without undergoing a “crisis” in a newly-developed serum-free medium, LEP/MK2. LEP/MK2 consists of calcium-free MEM with non-essential amino acids supplemented with 8 factors. Two lines, MK1 and MKDC4, have been isolated and have now doubled more than 400 and 200 times respectively. In MK1 cells, Giemsa banding has revealed significant karyotypic changes as early as the 4th passage, leading to a near-tetraploid karyotype… CONTINUE READING
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