Spontaneous ectopic recombination in cell-type-specific Cre mice removes loxP-flanked marker cassettes in vivo.

@article{Eckardt2004SpontaneousER,
  title={Spontaneous ectopic recombination in cell-type-specific Cre mice removes loxP-flanked marker cassettes in vivo.},
  author={Dominik Eckardt and Martin Theis and Britta D{\"o}ring and Dina Speidel and Klaus Willecke and Thomas Ott},
  journal={Genesis},
  year={2004},
  volume={38 4},
  pages={159-65}
}
Conditional gene targeting using the Cre/loxP technology generally includes integration of a selection marker cassette flanked by loxP recognition sites (floxed) in the target gene locus. Subsequent marker removal avoids possible impairment of gene expression or mosaicism due to partial and total deletions after Cre-mediated recombination in vivo. The use… CONTINUE READING