Spontaneous degradation of polypeptides at aspartyl and asparaginyl residues: effects of the solvent dielectric.


We have investigated the spontaneous degradation of aspartate and asparagine residues via succinimide intermediates in model peptides in organic co-solvents. We find that the rate of deamidation at asparagine residues is markedly reduced in solvents of low dielectric strength. Theoretical considerations suggest that this decrease in rate is due to the… (More)