Spontaneous chromosome doubling results from nuclear fusion during in vitro maize induced microspore embryogenesis

@article{Testillano2004SpontaneousCD,
  title={Spontaneous chromosome doubling results from nuclear fusion during in vitro maize induced microspore embryogenesis},
  author={Pilar S Testillano and Sevdalin Georgiev and H. Lloyd Mogensen and Mar{\'i}a Jos{\'e} Coronado and Christian Dumas and Mar{\'i}a Del Carmen Risue{\~n}o and Elisabeth Matthys-Rochon},
  journal={Chromosoma},
  year={2004},
  volume={112},
  pages={342-349}
}
A multidisciplinary study was carried out to analyse the chromosome doubling process during the early stages of in vitro maize microspore embryogenesis. The main stages (microspore derivatives) that were formed in the course of the culture were analysed. Chromosome number was determined from squashed cells, and DNA content was measured by cytometry. In parallel, an ultrastructural analysis of the microspore derivatives demonstrated the occurrence of a nuclear fusion process. It seems likely… CONTINUE READING