Spontaneous basophil responses in food-allergic children are transferable by plasma and are IgE-dependent.

  title={Spontaneous basophil responses in food-allergic children are transferable by plasma and are IgE-dependent.},
  author={John T. Schroeder and Anja P. Bieneman and Kristin L. Chichester and Corinne A. Keet and Robert G. Hamilton and Donald W MacGlashan and Robert A. B. Wood and Pamela A. Frischmeyer-Guerrerio},
  journal={The Journal of allergy and clinical immunology},
  volume={132 6},
To the Editor: Early studies showed that up to 80% of children with food allergy demonstrate high spontaneous basophil histamine release (SBHR).1 An association with IgE was suggested because basophils from food-allergic (FA) children additionally released histamine to the IgE-dependent histamine-releasing factor.2 However, the exact mechanism(s) responsible for SBHR, including the dependency for IgE, remains poorly understood. We recently reported that SBHR significantly decreased in… Expand
Suppression of the immunologic response to peanut during immunotherapy is often transient.
OIT and SLIT for peanut allergy induce rapid suppression of basophil effector functions, DC activation, and TH2 cytokine responses during the initial phases of immunotherapy in an antigen-nonspecific manner. Expand
Mechanistic correlates of clinical responses to omalizumab in the setting of oral immunotherapy for milk allergy
A combination of baseline basophil and serologic biomarkers defined a subset of patients in which adjunctive therapy with omalizumab was associated with attainment of sustained unresponsiveness and a reduction in adverse reactions. Expand
Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen
Findings point to a lectin-dependent activation of basophil requiring IgE but independent of allergen or secreted cytokine, which is linked to inflammatory conditions whereby IgE-dependentactivation of Basophils occurs despite the absence of any knownAllergen. Expand
Autoantibodies to IgE and Fc&egr;RI and the natural variability of spleen tyrosine kinase expression in basophils
  • D. MacGlashan
  • Medicine, Biology
  • The Journal of allergy and clinical immunology
  • 2019
It is established that functional autoantibodies to IgE/Fc&egr;RI do not provide a good explanation for the variability in SYK expression in basophils in the general population, and it is shown that if antibodies with these characteristics are present, they are capable of modulating SYKexpression in developing basophil. Expand
Histamine Release from Mast Cells and Basophils.
The in-depth knowledge of mast cell and basophil histamine release and its biologic effects is poised to uncover new therapeutic avenues for a wide spectrum of disorders. Expand
N‐glycan in cockroach allergen regulates human basophil function
The role of cockroach‐associated glycan in regulating human basophil function is examined in order to examine the mechanisms underlying the induction ofcockroach allergen‐induced allergy and asthma. Expand


Basophil reactivity, wheal size, and immunoglobulin levels distinguish degrees of cow's milk tolerance.
The majority of patients with milk allergy are able to tolerate some forms of baked milk in their diets and different phenotypes of children with cow's milk allergy can be distinguished by casein- and milk-specific IgE levels, milk- specific basophil reactivity, and milk SPT mean wheal diameters. Expand
Spontaneous release of histamine from basophils and histamine-releasing factor in patients with atopic dermatitis and food hypersensitivity.
It is concluded that in patients with food hypersensitivity, exposure to the relevant antigens produces a cytokine (histamine-releasing factor) that interacts with IgE bound to the surface of basophils, causing them to release histamine. Expand
Basophil CD203c Levels Are Increased at Baseline and Can Be Used to Monitor Omalizumab Treatment in Subjects with Nut Allergy
Subjects with nut allergy show an increase of basophil CD203c levels at baseline and following rapid ex vivo stimulation with nut allergen, and both can be reduced by omalizumab therapy. Expand
IgE-dependent IL-4 secretion by human basophils. The relationship between cytokine production and histamine release in mixed leukocyte cultures.
It appears that basophil IL-4 generation will have a proinflammatory effect, but it appears that the signal transduction mechanisms for its synthesis and release differ from those for histamine release and thus may require different methods of pharmacologic control. Expand
Assay Methods for Measurement of Mediators and Markers of Allergic Inflammation
With the discovery of immunoglobulin E (IgE) as the source of reaginic activity in serum, the release of histamine from leukocyte suspensions challenged with a specific antigen has been used as aExpand
High spontaneous release of histamine in vitro from leukocytes of persons hypersensitive to food.
  • C. May
  • Medicine
  • The Journal of allergy and clinical immunology
  • 1976
Of children proved by a double-blind food challenge to react with manifestations of immediate hypersensitivity, 100% had leukocytes that consistently released over 25% of the histamine content spontaneously without the addition of antigen. Expand
Dissociation of IgE from receptors on human basophils. I. Enhanced passive sensitization for histamine release.
Leukocytes of only one of 11 nonatopic donors could be passively sensitized for histamine release elicited by ragweed extract. A short incubation in an unbuffered isotonic saline at pH 3.9 or in anExpand
The safety and efficacy of sublingual and oral immunotherapy for milk allergy.
OIT was more efficacious for desensitization to CM than SLIT alone but was accompanied by more systemic side effects, and clinical desensITization was lost in some cases within 1 week off therapy. Expand
Cellular immune response parameters that influence IgE sensitization.
A review article takes a look at some of the recent advances in old and new assays employing both basophils and DCs, with the added perception that the responses mediated by two cell types are insightful towards understanding immune cell mechanisms underlying allergic disease. Expand
CD203c expression on human basophils is associated with asthma exacerbation.
Asthma exacerbation was accompanied by increased expression of CD 203c on basophils that decreased significantly during remission, and Basophil expression levels of CD203c might therefore be used to monitor asthma in patients. Expand