Spontaneous assembly of bivalent single chain antibody fragments in Escherichia coli.

@article{McGregor1994SpontaneousAO,
  title={Spontaneous assembly of bivalent single chain antibody fragments in Escherichia coli.},
  author={Duncan P McGregor and Peter E. Molloy and Charles Cunningham and William J. Harris},
  journal={Molecular immunology},
  year={1994},
  volume={31 3},
  pages={219-26}
}
The ability of immunoglobulin Fab and single chain (ScFv) fragments to penetrate effectively into tissue from the vascular system has made these molecules excellent candidates as drug delivery systems and imaging tools. This study investigates the use of single chain antibody fragment bacterial expression vectors as a possible strategy for the production of these molecules. We have modified the pSW1-VHD1.3-VKD1.3-TAG1 vector [Ward et al. (1989) Nature 341, 544-546] which originally, when… CONTINUE READING

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