Spontaneous and pokeweed mitogen-induced in vitro IgG production specific for S. aureus cell wall determinants in man.

@article{Hauser1984SpontaneousAP,
  title={Spontaneous and pokeweed mitogen-induced in vitro IgG production specific for S. aureus cell wall determinants in man.},
  author={Conrad Hauser and John A. Wilhelm and Lukas Matter and Kurt Schopfer},
  journal={Clinical and experimental immunology},
  year={1984},
  volume={57 1},
  pages={227-33}
}
A system has been established to produce in vitro IgG specific for cell wall determinants of S. aureus by human peripheral blood mononuclear cells (PBMC). PBMC of healthy individuals, of patients with S. aureus infections and of patients with other bacterial infections were cultured for 12 days. In the culture supernatants (SN) total IgG was determined by a competitive RIA, and IgG to purified cell walls (PCW) of S. aureus strain H by a two step ELISA. PBMC of 11 healthy persons produced anti… CONTINUE READING