Split single-cell RT-PCR analysis of Purkinje cells

@article{Esumi2006SplitSR,
  title={Split single-cell RT-PCR analysis of Purkinje cells},
  author={Shigeyuki Esumi and Ryosuke Kaneko and Yoshimi Kawamura and Takeshi Yagi},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={2143-2151}
}
This protocol details a method for analyzing the expression of multiple genes from a single Purkinje neuron, including the determination of whether the gene expression is monoallelic or biallelic. The protocol describes how to extract a single, living Purkinje cell for reverse transcription, divide the cDNAs into three equal samples and subject those to triplicate amplification of multiple targets by two rounds of PCR (first a multiplex PCR then a gene-specific nested PCR) and finally… CONTINUE READING
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