Split-sample comparison of directional and liquid nitrogen vapour freezing method on post-thaw semen quality in white rhinoceroses (Ceratotherium simum simum and Ceratotherium simum cottoni).

@article{Reid2009SplitsampleCO,
  title={Split-sample comparison of directional and liquid nitrogen vapour freezing method on post-thaw semen quality in white rhinoceroses (Ceratotherium simum simum and Ceratotherium simum cottoni).},
  author={C. E. Reid and R. Hermes and S. Blottner and F. Goeritz and G. Wibbelt and C. Walzer and B. Bryant and T. J. Portas and W. Streich and T. Hildebrandt},
  journal={Theriogenology},
  year={2009},
  volume={71 2},
  pages={
          275-91
        }
}
  • C. E. Reid, R. Hermes, +7 authors T. Hildebrandt
  • Published 2009
  • Biology, Medicine
  • Theriogenology
  • To increase the quality of cryopreserved sperm in white rhinoceros, the liquid nitrogen vapour (LN vapour) freezing and the multi-thermal gradient directional freezing methods were compared. Sixteen white rhinoceros (Ceratotherium simum sp.) were electro-ejaculated. Semen samples were diluted with cryoextender (Tris, lactose, egg-yolk, DMSO) and aliquoted into straws for LN vapour freezing, and glass hollow tubes for directional freezing. The sperm quality was evaluated before and after… CONTINUE READING
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