Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage.

@article{Lu2011SplitIF,
  title={Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage.},
  author={Wei Lu and Ziyong Sun and Yanchun Tang and Junyong Chen and Fengyuan Tang and Jing Zhang and J. Liu},
  journal={Journal of chromatography. A},
  year={2011},
  volume={1218 18},
  pages={2553-60}
}
Purification tags are robust tools that can be used to purify a variety of target proteins. However, tag removal remains an expensive and significant issue that must be resolved. Based on the affinity and the trans-splicing activity between the two domains of Ssp DnaB split-intein, a novel approach for tag affinity purification of recombinant proteins with controllable tag removal by inducible auto-cleavage has been developed. This system provides a new affinity method and avoids premature… CONTINUE READING
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