Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.

@article{Romei2019SplitGF,
  title={Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.},
  author={Matthew G. Romei and Steven G Boxer},
  journal={Annual review of biophysics},
  year={2019},
  volume={48},
  pages={
          19-44
        }
}
Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein-protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies. 

Figures, Tables, and Topics from this paper.

Citations

Publications citing this paper.

References

Publications referenced by this paper.
SHOWING 1-10 OF 159 REFERENCES

A Genetically Encodable System for Sequence-Specific Detection of RNAs in Two Colors.

  • Chembiochem : a European journal of chemical biology
  • 2016
VIEW 6 EXCERPTS
HIGHLY INFLUENTIAL

ON THE ENZYMIC ACTIVITY OF SUBTILISIN-MODIFIED RIBONUCLEASE.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 1958
VIEW 8 EXCERPTS
HIGHLY INFLUENTIAL