Split-Cre Complementation Indicates Coincident Activity of Different Genes In Vivo

@article{Hirrlinger2009SplitCreCI,
  title={Split-Cre Complementation Indicates Coincident Activity of Different Genes In Vivo},
  author={Johannes Hirrlinger and Anja Scheller and Petra G. Hirrlinger and Beate Kellert and Wannan Tang and Michael C Wehr and Sandra Goebbels and Andreas Reichenbach and Rolf Sprengel and Moritz J Rossner and Frank Kirchhoff},
  journal={PLoS ONE},
  year={2009},
  volume={4},
  pages={99 - 109}
}
Cre/LoxP recombination is the gold standard for conditional gene regulation in mice in vivo. However, promoters driving the expression of Cre recombinase are often active in a wide range of cell types and therefore unsuited to target more specific subsets of cells. To overcome this limitation, we designed inactive "split-Cre" fragments that regain Cre activity when overlapping co-expression is controlled by two different promoters. Using transgenic mice and virus-mediated expression of split… CONTINUE READING

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