Splinted ligation method to detect small RNAs.

  title={Splinted ligation method to detect small RNAs.},
  author={Timothy W Nilsen},
  journal={Cold Spring Harbor protocols},
  volume={2013 1}
In any cell, the number of RNA species is remarkably complex. The sizes of RNAs can vary from 20 nucleotides to several kilobases, and abundances can vary from a few to hundreds of thousands of molecules per cell. It is of obvious interest to determine the abundance and integrity of specific RNA species within these complex mixtures. This protocol describes the splinted ligation method to detect small RNAs. It relies on the ability of T4 DNA ligase to covalently join the terminal 3'-hydroxyl… CONTINUE READING

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