Splicing promotes the nuclear export of β-globin mRNA by overcoming nuclear retention elements.

@article{Akef2015SplicingPT,
  title={Splicing promotes the nuclear export of $\beta$-globin mRNA by overcoming nuclear retention elements.},
  author={Abdalla Akef and Eliza S. Lee and Alexander F Palazzo},
  journal={RNA},
  year={2015},
  volume={21 11},
  pages={
          1908-20
        }
}
Most current models of mRNA nuclear export in vertebrate cells assume that an mRNA must have specialized signals in order to be exported from the nucleus. Under such a scenario, mRNAs that lack these specialized signals would be shunted into a default pathway where they are retained in the nucleus and eventually degraded. These ideas were based on the selective use of model mRNA reporters. For example, it has been shown that splicing promotes the nuclear export of certain model mRNAs, such as… Expand
Sequence Determinants for Nuclear Retention and Cytoplasmic Export of mRNAs and lncRNAs
TLDR
A new understanding of the determinants of nuclear retention and cytoplasmic export provides a deeper understanding of how information flow is regulated in eukaryotic cells. Expand
TPR is required for the efficient nuclear export of mRNAs and lncRNAs from short and intron-poor genes
TLDR
Analysis of mRNA from the nucleus and cytosol of control and TPR-depleted cells provides evidence that TPR is required for the efficient nuclear export of mRNAs and lncRNAs that are generated from short transcripts that tend to have few introns, and validate this with reporter constructs. Expand
TPR is required for the nuclear export of mRNAs and lncRNAs from intronless and intron-poor genes
TLDR
Analysis of mRNA from the nucleus and cytosol of control and TPR-depleted cells provides evidence that TPR is required for the nuclear export of mRNAs and lncRNAs that are generated from intronless and intron-poor genes, and validate this with reporter constructs. Expand
Nuclear sorting of RNA
TLDR
This review details the current understanding of the pathways and factors that direct RNAs towards a particular destiny and how transcripts combat the adverse conditions of the nucleus. Expand
A nuclear zip code in SKS1 mRNA promotes its slow export, nuclear retention, and degradation by the nuclear exosome/DRN in Saccharomyces cerevisiae.
TLDR
A cis-acting zip code element is identified in SKS1 mRNA that is necessary and sufficient to impede nuclear export and results in its preferential nuclear retention, thereby impacting its abundance and cellular repertoire. Expand
ZFC3H1 and U1-70K promote the nuclear retention of mRNAs with 5’ splice site motif within nuclear speckles
TLDR
This work demonstrates that mature mRNAs containing a 5’ splice site (5’SS) motif, which is typically found in misprocessed RNAs such as intronic polyadenylated (IPA) transcripts, are nuclear retained and degraded and suggests a model where U1 snRNP acts with ZFC3H1 to both promote their decay and prevent nuclear export of these m RNAs by sequestering them in nuclear speckles. Expand
Intronless β-Globin Reporter: A Tool for Studying Nuclear RNA Stability Elements.
TLDR
This chapter explains how to perform the β-globin reporter assay using the ENE (expression and nuclear retention element), a triple-helix-forming RNA stability element that protects reporter mRNA from 3'- 5' decay. Expand
Gene Architecture and Sequence Composition Underpin Selective Dependency of Nuclear Export of Long RNAs on NXF1 and the TREX Complex.
TLDR
The core components of the nuclear RNA export pathway are thought to be required for export of virtually all polyadenylated RNAs, and depletion of NXF1 and TREX components causing some transcripts to become strongly retained in the nucleus while others were not affected. Expand
Sequences enriched in Alu repeats drive nuclear localization of long RNAs in human cells
TLDR
A pathway for regulation of RNA accumulation and subcellular localization that has been co-opted to regulate the fate of transcripts with integrated Alu elements is identified. Expand
Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway
TLDR
It is concluded that the binding of SRSF1/7 to clustered motifs in CAR-N facilitated TREX recruitment, promoting the export of NKILA, and confirmed the importance ofNKILA localization to its function. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 49 REFERENCES
The Signal Sequence Coding Region Promotes Nuclear Export of mRNA
TLDR
The surprising observation that the signal sequence coding region (SSCR) can serve as a nuclear export signal of an mRNA that lacks an intron or functional cap is made and suggests a link between nuclear export and membrane targeting of mRNAs. Expand
The Consensus 5' Splice Site Motif Inhibits mRNA Nuclear Export
TLDR
The data suggests that the presence of the 5’splice site motif in mature RNAs promotes their nuclear retention and may help to distinguish mRNAs from misprocessed transcripts and transcriptional noise. Expand
REF proteins mediate the export of spliced and unspliced mRNAs from the nucleus.
TLDR
The REF family of evolutionarily conserved heterogeneous ribonucleoprotein (hnRNP)-like proteins consists of one central RNP-type RNA binding domain flanked by Arg-Gly-rich regions of variable length, and data indicate that REFs are directly implicated in the export of mRNAs from the nucleus. Expand
Splicing is required for rapid and efficient mRNA export in metazoans.
  • M. Luo, R. Reed
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1999
TLDR
A link between splicing and efficient mRNA export is revealed, which may explain the reports that an intron is required for efficient expression of many protein-coding genes in metazoans. Expand
Analysis of the stimulatory effect of splicing on mRNA production and utilization in mammalian cells.
TLDR
It is argued that splicing can increase gene expression by enhancing mRNA 3' end processing, and hence, mRNA production, in the case of the highly intron-dependent beta-globin gene, which significantly enhanced the translational utilization of cytoplasmic beta- globin mRNAs. Expand
Splicing promotes rapid and efficient mRNA export in mammalian cells
TLDR
It is concluded that splicing promotes mRNA export in mammalian cells and that the functional coupling between splicing and mRNA export is a conserved and general feature of gene expression in higher eukaryotes. Expand
ALREX‐elements and introns: two identity elements that promote mRNA nuclear export
TLDR
The current knowledge of how mRNA export evolved in response to particular events that occurred at the base of the eukaryotic tree is reviewed and the current understanding of ALREX is compared to splicing‐dependent export, the main mRNA export pathway in metazoans. Expand
Export and stability of naturally intronless mRNAs require specific coding region sequences and the TREX mRNA export complex
TLDR
It is concluded that naturally intronless mRNAs contain specific sequences that result in efficient packaging into the TREX mRNA export complex, thereby supplanting the splicing requirement for efficient mRNA export. Expand
The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans
TLDR
It is shown that Aly, the metazoan homologue of the yeast mRNA export factor Yra1p, is recruited to messenger ribonucleoprotein (mRNP) complexes generated by splicing. Expand
Human mRNA Export Machinery Recruited to the 5′ End of mRNA
TLDR
Data indicate that the CBP80-Aly interaction results in recruitment of TREX to the 5' end of mRNA, where it functions in mRNA export, as observed in early electron micrographs of giant Balbiani ring mRNPs. Expand
...
1
2
3
4
5
...