Splicing double: insights from the second spliceosome
@article{Patel2003SplicingDI, title={Splicing double: insights from the second spliceosome}, author={Abhijit A. Patel and Joan A. Steitz}, journal={Nature Reviews Molecular Cell Biology}, year={2003}, volume={4}, pages={960-970} }
Almost 20 years after the discovery of introns and RNA splicing, a second spliceosome was uncovered. Although this new spliceosome is structurally and functionally analogous to the well-characterized major-class splicing apparatus, it mediates the excision of a minor class of evolutionarily conserved introns that have non-canonical consensus sequences. This unanticipated diversity in the splicing machinery is refining both the mechanistic understanding and evolutionary models of RNA splicing.
441 Citations
Splicing of a rare class of introns by the U12-dependent spliceosome
- BiologyBiological chemistry
- 2005
This review focuses on recent advances in elucidating the structure and function of the minor U12-dependent spliceosome.
Spliceosome assembly and composition.
- BiologyAdvances in experimental medicine and biology
- 2007
This chapter discusses recent advances regarding similarities and differences between the in vivo and in vitro assembly pathways, as well as proofreading mechanisms contributing to the fidelity of splice site selection.
Targeted ‘knockdown’ of spliceosome function in mammalian cells
- BiologyNucleic acids research
- 2005
It is shown that morpholino oligomers complementary to the branch-site recognition elements of U2 or U12 small nuclear RNA specifically suppress the function of the two splicing systems in mammalian cells.
Regulation of gene expression through inefficient splicing of U12-type introns
- BiologyRNA biology
- 2014
The potential implications of recent genome-wide studies on the splicing of U12-type introns, including human patient cells with mutations in the components of the minor spliceosome, zebrafish with similar mutations and various experimentally manipulated human cells and Arabidopsis plants are discussed.
Splicing of U12-type introns deposits an exon junction complex competent to induce nonsense-mediated mRNA decay
- BiologyProceedings of the National Academy of Sciences of the United States of America
- 2004
In vitro splicing of a U12-type intron assembles an exon junction complex (EJC) that is comparably positioned and contains many of the same components as that deposited by the U2-type spliceosome.
Alternative splicing in ascomycetes
- BiologyApplied Microbiology and Biotechnology
- 2013
This review focuses onAlternative splicing in ascomycetes, which in general have significant lower extend of alternative splicing than mammals and putative protein factors involved in regulation.
References
SHOWING 1-10 OF 141 REFERENCES
Pre-mRNA splicing: the discovery of a new spliceosome doubles the challenge.
- BiologyTrends in biochemical sciences
- 1997
Self-splicing group II and nuclear pre-mRNA introns: how similar are they?
- Biology, PhysicsTrends in biochemical sciences
- 1990
Initial splice-site recognition and pairing during pre-mRNA splicing.
- BiologyCurrent opinion in genetics & development
- 1996
A Parallel Spliceosome
- BiologyScience
- 1996
In his Perspective, Nilsen describes why the now complete description of the RNA components of this spliceosome has larger implications for splicing mechanisms in general.
Spliceosomal UsnRNP biogenesis, structure and function.
- BiologyCurrent opinion in cell biology
- 2001
Spliceosomal RNA U6 is remarkably conserved from yeast to mammals
- BiologyNature
- 1988
An extensive base-pairing interaction with U4 snRNA is described; the destabilization of the U4/U6 complex seen during splicing thus requires a large conformational change.
Mechanisms of alternative pre-messenger RNA splicing.
- BiologyAnnual review of biochemistry
- 2003
This review describes what is currently known of the molecular mechanisms that control changes in splice site choice and starts with the best-characterized systems from the Drosophila sex determination pathway, and then describes the regulators of other systems about whose mechanisms there is some data.
Alternative pre-mRNA splicing: the logic of combinatorial control.
- BiologyTrends in biochemical sciences
- 2000