Spliceosomal U snRNP core assembly: Sm proteins assemble onto an Sm site RNA nonanucleotide in a specific and thermodynamically stable manner.

@article{Raker1999SpliceosomalUS,
  title={Spliceosomal U snRNP core assembly: Sm proteins assemble onto an Sm site RNA nonanucleotide in a specific and thermodynamically stable manner.},
  author={Veronica A. Raker and Klaus Hartmuth and Berthold Kastner and Reinhard L{\"u}hrmann},
  journal={Molecular and cellular biology},
  year={1999},
  volume={19 10},
  pages={6554-65}
}
The association of Sm proteins with U small nuclear RNA (snRNA) requires the single-stranded Sm site (PuAU(4-6)GPu) but also is influenced by nonconserved flanking RNA structural elements. Here we demonstrate that a nonameric Sm site RNA oligonucleotide sufficed for sequence-specific assembly of a minimal core ribonucleoprotein (RNP), which contained all… CONTINUE READING