Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency

  title={Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency},
  author={Ignacio Garc{\'i}a-Tu{\~n}{\'o}n and Ver{\'o}nica Alonso-P{\'e}rez and Elena Vuelta and Sandra P{\'e}rez-Ramos and Mar{\'i}a Herrero and Luc{\'i}a M{\'e}ndez and Jes{\'u}s Mar{\'i}a Hern{\'a}ndez-S{\'a}nchez and Marta Mart{\'i}n-Izquierdo and R. M. Castro Salda{\~n}a and Juli{\'a}n Sevilla and Ferm{\'i}n S{\'a}nchez-Guijo and Jesus M. Hernandez-Rivas and Manuel S{\'a}nchez-Mart{\'i}n},
  booktitle={PloS one},
CRISPR/Cas9 allows the generation of knockout cell lines and null zygotes by inducing site-specific double-stranded breaks. In most cases the DSB is repaired by non-homologous end joining, resulting in small nucleotide insertions or deletions that can be used to construct knockout alleles. However, these mutations do not produce the desired null result in all cases, but instead generate a similar, functionally active protein. This effect could limit the therapeutic efficiency of gene therapy… CONTINUE READING

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