Splenda Alters Gut Microflora and Increases Intestinal P-Glycoprotein and Cytochrome P-450 in Male Rats

  title={Splenda Alters Gut Microflora and Increases Intestinal P-Glycoprotein and Cytochrome P-450 in Male Rats},
  author={Mohamed B. Abou‐Donia and Eman M. El-Masry and Ali A. Abdel-Rahman and Roger E. McLendon and Susan S. Schiffman},
  journal={Journal of Toxicology and Environmental Health, Part A},
  pages={1415 - 1429}
Splenda is comprised of the high-potency artificial sweetener sucralose (1.1%) and the fillers maltodextrin and glucose. Splenda was administered by oral gavage at 100, 300, 500, or 1000 mg/kg to male Sprague-Dawley rats for 12-wk, during which fecal samples were collected weekly for bacterial analysis and measurement of fecal pH. After 12-wk, half of the animals from each treatment group were sacrificed to determine the intestinal expression of the membrane efflux transporter P-glycoprotein (P… 

Intestinal Metabolism and Bioaccumulation of Sucralose In Adipose Tissue In The Rat

New findings of metabolism of sucralose in the gastrointestinal tract (GIT) and its accumulation in adipose tissue were not part of the original regulatory decision process for this agent and indicate that it now may be time to revisit the safety and regulatory status of this organochlorine artificial sweetener.

Expert panel report on a study of Splenda in male rats.

Low Dose of Sucralose Alter Gut Microbiome in Mice

It is indicated that low dose of sucralose (T1) alter gut microbiome in mice, and these adverse health effects are equal to ADI level (T4), which provides guidance and suggestions for the use of Sucralose in foods and beverages.

Ten-Week Sucralose Consumption Induces Gut Dysbiosis and Altered Glucose and Insulin Levels in Healthy Young Adults

Examination of the effect of sucralose consumption on the intestinal abundance of bacterial species belonging to Actinobacteria, Bacteroidetes, and Firmicutes and potential associations between microbiome profiles and glucose and insulin blood levels in healthy young adults revealed that volunteers drinking sucralOSE for ten weeks showed a 3-fold increase in Blautia coccoides and a 0.66-fold decrease in Lactobacillus acidophilus.

Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats

The effects of lower doses of sucralose on fecal microbiota in obesity were investigated and the relative abundance of family Acidaminoccaceae and its genus Phascolarctobacteriam were increased and the richness and diversity of fecal bacteria were not changed.

Sucralose, A Synthetic Organochlorine Sweetener: Overview of Biological Issues

  • S. SchiffmanK. Rother
  • Biology
    Journal of toxicology and environmental health. Part B, Critical reviews
  • 2013
Both human and rodent studies demonstrated that sucralose may alter glucose, insulin, and glucagon-like peptide 1 (GLP-1) levels, indicating that Sucralose is not a biologically inert compound.

Daikenchuto (TU‐100) alters murine hepatic and intestinal drug metabolizing enzymes in an in vivo dietary model: effects of gender and withdrawal

Daikenchuto (TU‐100), an aqueous extract of ginger, ginseng, and Japanese green pepper fruit, is a commonly prescribed Kampo (Japanese herbal medicine) for postoperative ileus or bloating and its effects on drug metabolism are investigated.

Metagenomic Analyses of Alcohol Induced Pathogenic Alterations in the Intestinal Microbiome and the Effect of Lactobacillus rhamnosus GG Treatment

Overall, significant alterations in the gut microbiome over time occur in response to chronic alcohol exposure and correspond to increases in intestinal barrier dysfunction and development of ALD.

The Artificial Sweetener Splenda Promotes Gut Proteobacteria, Dysbiosis, and Myeloperoxidase Reactivity in Crohn’s Disease–Like Ileitis

The results indicate that although Splenda may promote parallel microbiome alterations inCD-prone and healthy hosts, this did not result in elevated MPO levels in healthy mice, only CD-prone mice.

Non-nutritive sweeteners possess a bacteriostatic effect and alter gut microbiota in mice

The data show that NNSs have direct bacteriostatic effects and can change the intestinal microbiota in vivo.



A carcinogenicity study of sucralose in the CD-1 mouse.

The role of intestinal bacteria in the metabolism of salicylazosulfapyridine.

Findings indicate that tile intestinal bacteria are responsible for the initial reaction in SAS transformation and raise questions concerning the amount of intact SAS that reaches the presumed site of its action in inflammatory disease of the lower intestine.

The metabolic fate of sucralose in rats.

  • J. SimsA. RobertsJ. DanielA. Renwick
  • Medicine, Biology
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2000

Role of intestinal bacteria in nutrient metabolism.

Collaborative JPEN‐Clinical Nutrition Scientific Publications Role of intestinal bacteria in nutrient metabolism

The human large intestine contains a microbiota, the components of which are generically complex and metabolically diverse, and the amount of protein synthesis and turnover within the large intestine is difficult to determine.

Chlorpyrifos oxon interacts with the mammalian multidrug resistance protein, P-glycoprotein.

The hypothesis that P-gp may play a role in the cellular detoxification of insecticides in mammalian tissues is supported after the first report of an organophosphorus insecticide interacting with and increasing the expression of P- gp.

Role of P-glycoprotein and cytochrome P450 3A in limiting oral absorption of peptides and peptidomimetics.

There is relatively little data regarding the effects of CYP3A and P-gp on peptide drugs; however, studies with the cyclic peptide immunosuppresant cyclosporine as well as peptidomimetics provide some insight into the impact of these systems on the oral absorption of peptides.

The gut microbiota as an environmental factor that regulates fat storage.

It is found that conventionalization of adult germ-free (GF) C57BL/6 mice with a normal microbiota harvested from the distal intestine of conventionally raised animals produces a 60% increase in body fat content and insulin resistance within 14 days despite reduced food intake.

Feeding resistant starch affects fecal and cecal microflora and short-chain fatty acids in rats.

Stimulation of bifidobacteria, lactobacilli, and SCFA may be useful for the suppression of pathogenic organisms in the colon in rats fed RS 1 or control rats.

Probiotics as modulators of the gut flora

The approach of modulating the gut flora for improved health has much relevance for the management of those with acute and chronic gut disorders, as well as the elderly, who have an altered microflora, with a decreased number of beneficial microbial species.