Spleen neoangiogenesis in patients with myelofibrosis with myeloid metaplasia.


Neoangiogenesis is an integral component of bone marrow myeloproliferation in patients with myelofibrosis with myeloid metaplasia (MMM). As extramedullary haematopoiesis is a constitutive feature of MMM, we studied spleen neoangiogenesis by a computerized image analysis in MMM patients. Compared with five normal subjects, spleen CD34-staining capillary vascular density (CVD) was 2.1-3.03 times higher than the upper range of normal in six of the 15 (40%) MMM patients. CD8-staining sinusoidal vascular density (SVD) was constantly normal or lesser than normal and was inversely correlated with CVD (R = -0.53; P = 0.04). In MMM patients who did not receive cytoreductive or radiation therapy in the month before splenectomy (n = 9), the CVD was a significant determinant of spleen size (R = 0.88; P = 0.04). In MMM patients, the number of spleen CD34+ haematopoietic stem cells was increased from 1.2 to 98 times the upper limit of normal, and predicted the expansion of CVD (R = 0.57; P = 0.03). A population of cells expressing the CD34+/CD133+/VEGFR-2+ angiopoietic phenotype was present in the blood and spleen of five of seven patients. These results document that neoangiogenesis is an integral component of spleen re-localization of haematopoietic stem cells and suggest a cellular mechanism for spleen neoangiogenesis.

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@article{Barosi2004SpleenNI, title={Spleen neoangiogenesis in patients with myelofibrosis with myeloid metaplasia.}, author={Giovanni Barosi and Vittorio Rosti and Margherita Massa and Gian Luca Viarengo and Alessandro Pecci and Vittorio Necchi and Isabella Ramaioli and Rita Campanelli and Monia Marchetti and M. Bazzan and Umberto Magrini}, journal={British journal of haematology}, year={2004}, volume={124 5}, pages={618-25} }