Spiroplasma-like organisms closely associated with the gut in five leafhopper species (Hemiptera: Cicadellidae)

  title={Spiroplasma-like organisms closely associated with the gut in five leafhopper species (Hemiptera: Cicadellidae)},
  author={El Desouky Ammar and Gail E. Gasparich and David G. Hall and Saskia A. Hogenhout},
  journal={Archives of Microbiology},
Spiroplasmas are bacteria in the Class Mollicutes that are frequently associated with insects and/or plants. Here, we describe the ultrastructure, localization, and occurrence of apparent commensal/symbiotic spiroplasma-like organisms (SLOs) in the midgut and hindgut of five leafhopper species from laboratory-reared colonies. Those found in Dalbulus elimatus, Endria inimica, and Macrosteles quadrilineatus were long and tubular shaped, whereas those in Dalbulus maidis and Graminella nigrifrons… 

Bacterial diversity of bacteriomes and organs of reproductive, digestive and excretory systems in two cicada species (Hemiptera: Cicadidae)

Bacterial communities in the bacteriomes and organs of reproductive, digestive and excretory systems of two cicada species were investigated using different methods, and the bacterial diversity and distribution patterns of dominant bacteria in different tissues were compared.

Invasion of insect cells by Spiroplasma citri involves spiralin relocalization and lectin/glycoconjugate‐type interactions

Evidence is provided that the lipoprotein spiralin plays a major role in the very early step of cell invasion and competitive adhesion assays with lectins strongly suggest spiralin to exhibit glycoconjugate binding properties similar to that of the Vicia villosa agglutinin (VVA) lectin.

Microbe Relationships with Phytoplasmas in Plants and Insects

Here, many bacteria, mainly of the genera Pseudomonas, Burkholderia and Paenibacillus, as well as the fungal endophyte Epicoccum nigrum, were shown to inhibit phytoplasma growth and related symptoms in the plant hosts.

The dynamics of biological Russian dolls: investigating the causes and consequences of variation in symbiont density in citrus mealybugs

Symbiont density was not found to correlate with life-history traits in the laboratory, the ability of mealybug to exploit different plant species, or the susceptibility of the mealybugs to insecticide and artificial reduction of symbiontdensity by heat-stress also had no effect on host fitness.

Interactions of maize bushy stunt phytoplasma with the leafhopper vector, Dalbulus maidis (Delong and Wolcott) (Hemiptera: Cicadellidae) and associated microbiota

Transmission electron microscopy analyses suggest that MBSP modulates D. maidis preference for asymptomatic infected plants in the early stages of the crop, allowing rapid spread of this pathogen.

Geographic Variation of Bacterial Communities Associated with Cotton Fleahopper, Pseudatomoscelis seriatus1

Characterization of the P. seriatus bacterial microbiota showed it was distinct from other well-characterized hemipteran herbivores, and whether variation in microbiota corresponds with variation in traits relevant to pest control warrants further investigation.



Spiroplasma citri, a plant pathogenic molligute: relationships with its two hosts, the plant and the leafhopper vector.

Spiroplasma citri, the type species of the genus Spiroplasma (Spiroplasmataceae, Mollicutes), is restricted to the phloem sieve tubes and transmitted by phloem sap-feeding insects, as is

Spiroplasmas: infectious agents of plants, arthropods and vertebrates.

  • J. Bové
  • Biology
    Wiener klinische Wochenschrift
  • 1997
Following the pioneering work on S. citri and S. kunkelii, close to fifty other spiroplasma species or proposed species have been discovered and a non-motile mutant has been obtained to study involvement of spiroPLasmal motility in pathogenicity.

Spiroplasma leptinotarsae sp. nov., a Mollicute Uniquely Adapted to Its Host, the Colorado Potato Beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

The results of extensive studies of the ecology of this spiroplasma suggest that it is host specific for Leptinotarsa beetles.

Spiroplasma citri Movement into the Intestines and Salivary Glands of Its Leafhopper Vector, Circulifer tenellus.

Cytopathological effects of spiroplasma infection in salivary cells included loss of membrane and basal lamina integrity, presence in some cells of irregular inclusion-like structures containing dense matrices of filamentous material that labeled with anti S. citri antibodies, and apparent disorganization of the endoplasmic reticulum.

An attachment tip and pili-like structures in insect- and plant-pathogenic spiroplasmas of the class Mollicutes

Findings suggest that the tip structure may be involved in the orientation and attachment of spiroplasma helices in relation to their host cells, and thus may be functionally comparable to the “attachment organelle” of mycoplasmas.

Bacterial Communities within Digestive Tracts of Ground Beetles (Coleoptera: Carabidae)

The identification of bacteria uniquely and consistently associated with these ground beetles provides the basis for further investigation of species-specific functional roles.

Diversity and geographic distribution of secondary endosymbiotic bacteria in natural populations of the pea aphid, Acyrthosiphon pisum

It was demonstrated that different S‐symbionts coexist commonly in the same local populations, but double infections with two S‐ SYMBionts were rarely detected, and the distribution of PAUS infection might be related to host plant species, temperature and precipitation.

The genus Spiroplasma and its non-helical descendants: phylogenetic classification, correlation with phenotype and roots of the Mycoplasma mycoides clade.

A detailed phylogenetic study of Spiroplasma and its non-helical descendants was undertaken, and a new nomenclature is introduced here, based on 'bottom-up' rather than 'top-down' clade classification.

Use of Immunofluorescence Confocal Laser Scanning Microscopy to Study Distribution of the Bacterium Corn Stunt Spiroplasma in Vector Leafhoppers (Hemiptera: Cicadellidae) and in Host Plants

Compared with epifluorescence microscopy, iCLSM provides three-dimensional images of the studied organs, indicating their spatial relationships, which can be valuable for studying the routes of pathogens in their vectors.