Spindle assembly: asters part their separate ways

  title={Spindle assembly: asters part their separate ways},
  author={Jody Rosenblatt},
  journal={Nature Cell Biology},
  • J. Rosenblatt
  • Published 1 March 2005
  • Biology, Physics
  • Nature Cell Biology
Cells have developed diverse ways to separate two microtubule asters to form a mitotic spindle. Here, I focus on two mechanisms used to position asters around chromosomes during mitosis: first, aster migration around the nuclear envelope and, second, aster attachment to a contractile cortex at the plasma membrane after the nuclear envelope has broken down. Although certain cell types use one mechanism predominantly, most rely on both to ensure proper spindle assembly. 

Microtubule assembly during mitosis – from distinct origins to distinct functions?

Recent advances in the field are reviewed and how different MT assembly pathways might cooperate to successfully form the mitotic spindle is discussed.

Mechanisms of mitotic spindle assembly and function.

Orchestrating nuclear envelope disassembly and reassembly during mitosis

These studies reveal a tight interplay between NE components and the mitotic machinery in eukaryotic cell division.

Centrosomes and the art of mitotic spindle maintenance.

  • E. Hinchcliffe
  • Biology
    International review of cell and molecular biology
  • 2014

Kif18B interacts with EB1 and controls astral microtubule length during mitosis

Kif18B is a newly discovered plus-tip-tracking protein that is enriched on astral microtubule (MT) ends during early mitosis. Kif18B binds directly to EB1, and this interaction is required for proper

Amphiastral Mitotic Spindle Assembly in Vertebrate Cells Lacking Centrosomes

Timing of centrosome separation is important for accurate chromosome segregation

A combination of experimental and computational approaches shows that incomplete centrosome separation at NEB decreases the accuracy of chromosome segregation and thus represents a severe threat to genome stability.

Bicaudal D2, Dynein, and Kinesin-1 Associate with Nuclear Pore Complexes and Regulate Centrosome and Nuclear Positioning during Mitotic Entry

Mammalian Bicaudal D2 is the missing molecular link between cytoplasmic motor proteins and the nucleus during nuclear positioning prior to the onset of mitosis.



Microtubule motors in mitosis

It is becoming clear that motors invoke several distinct mechanisms to generate the forces that drive mitosis, and in carrying out its function, the spindle appears to pass through a series of transient steady-state structures, each established by a delicate balance of forces generated by multiple complementary and antagonistic motors.

The Mitotic Spindle: A Self-Made Machine

The emerging picture is that spindle assembly is governed by a combination of modular principles and that their relative contribution may vary in different cell types and in various organisms.

Microtubule-based motor function in mitosis.

Mitosis Through the Microscope: Advances in Seeing Inside Live Dividing Cells

The most visually spectacular events in the life of a cell occur when it divides. This is especially true in higher eukaryotes, where the size and geometry of cells allow the division process to be

Correcting improper chromosome–spindle attachments during cell division

By removal of small-molecule inhibitors, controlled activation of Aurora kinase during mitosis can correct chromosome attachment errors by selective disassembly of kinetochore–microtubule fibres, rather than by alternative mechanisms involving initial release of microtubules from either Kinetochores or spindle poles.

Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis

It is proposed that the capture and incorporation of preformed K-fibers complements the microtubule plus-end capture mechanism and contributes to spindle formation in vertebrates.

Mitotic spindle organization by a plus-end-directed microtubule motor

An in vitro assay for spindle assembly, derived from Xenopus egg extracts, is used to investigate the role of Eg5, a kinesin-like protein in Xenopus eggs, and demonstrates that Eg5 is a plus-end-directed microtubule motor in vitro.