Spin-labelling of phosphorylase b using a paramagnetic 1-fluoro-2,4-dinitrobenzene derivative.

Abstract

Phosphorylase b (1,4-alpha-D-glucan:1,6-alpha-D-glucan 6-alpha-glucosyltransferase, EC 2.4.1.1) can be specifically spin-labelled at a site essential for the catalytic action of the enzyme. A paramagnetic analogue of 1-fluoro-2,4-dinitrobenzene was synthesized and used as a dinitrophenylating agent. Reaction of phosphorylase b with the paramagnetic probe… (More)

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Cite this paper

@article{Cazianis1980SpinlabellingOP, title={Spin-labelling of phosphorylase b using a paramagnetic 1-fluoro-2,4-dinitrobenzene derivative.}, author={C T Cazianis and Theodore G. Sotiroudis and Apostolos E A S Evangelopoulos}, journal={Biochimica et biophysica acta}, year={1980}, volume={621 1}, pages={117-29} }