Spin-label Studies of Membranes in Rye Protoplasts during Extracellular Freezingl 2


Protoplasts isolated from epicotyls of nonhardened winter rye seedlings were spin-labeled with the N-oxyl44dimethyloxazolidine derivatives of 5ketostearic (5NS) and 16-ketostearic (16NS) acids. Spectra of the membrane-bound labels showed motional broadening with a rotational correlation time of 1.5 x 10'second for 5NS and 1.5 x 10-10 second for 16NS at 0 C… (More)

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