Spiking of contemporary human template DNA with ancient DNA extracts induces mutations under PCR and generates nonauthentic mitochondrial sequences.

@article{Pusch2004SpikingOC,
  title={Spiking of contemporary human template DNA with ancient DNA extracts induces mutations under PCR and generates nonauthentic mitochondrial sequences.},
  author={Carsten Matthias Pusch and Lutz Bachmann},
  journal={Molecular biology and evolution},
  year={2004},
  volume={21 5},
  pages={957-64}
}
Proof of authenticity is the greatest challenge in palaeogenetic research, and many safeguards have become standard routine in laboratories specialized on ancient DNA research. Here we describe an as-yet unknown source of artifacts that will require special attention in the future. We show that ancient DNA extracts on their own can have an inhibitory and mutagenic effect under PCR. We have spiked PCR reactions including known human test DNA with 14 selected ancient DNA extracts from human and… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 18 extracted citations

Ancient DNA

Russian Journal of Genetics • 2006
View 5 Excerpts
Highly Influenced

References

Publications referenced by this paper.

Similar Papers

Loading similar papers…