Spider Web DNA: A New Spin on Noninvasive Genetics of Predator and Prey

@article{Xu2014SpiderWD,
  title={Spider Web DNA: A New Spin on Noninvasive Genetics of Predator and Prey},
  author={Charles C. Y. Xu and Ivy J. Yen and Dean Bowman and Cameron R. Turner},
  journal={PLoS ONE},
  year={2014},
  volume={10}
}
Noninvasive genetic approaches enable biomonitoring without the need to directly observe or disturb target organisms. Environmental DNA (eDNA) methods have recently extended this approach by assaying genetic material within bulk environmental samples without a priori knowledge about the presence of target biological material. This paper describes a novel and promising source of noninvasive spider DNA and insect eDNA from spider webs. Using black widow spiders (Latrodectus spp.) fed with house… 

Figures and Tables from this paper

DNA extraction from spider webs

The successful isolation of target DNA from members of two families using a standard CTAB phenol–chloroform–isoamyl protocol adds to other studies in demonstrating that webbing offers an excellent resource for genetic studies of spiders across families.

Spider webs as eDNA samplers: Biodiversity assessment across the tree of life

The results show that genetic remains on spider webs allow the detection of even the smallest target organisms, and demonstrate that eDNA from spider webs is useful in research of community compositions across the different domains of life, with potentially highly detailed temporal and spatial information.

Spider webs as eDNA tool for biodiversity assessment of life’s domains

The results show that genetic remains on spider webs allow the detection of even the smallest target organisms and demonstrate that eDNA from spider webs is useful in research of community compositions in different domains of life, with potentially highly detailed temporal and spatial information.

A new primer for metabarcoding of spider gut contents

As a key predator group, spiders have received a lot of attention by food web ecologists in diverse fields such as pest control, pollutant transfers, and cross-ecosystem fluxes. The difficulty

Sampling Spider Webs for the Detection of Black Widow DNA

It is expected that some, but not all, of the webs Sampling Spider Webs for the Detection of Black Widow DNA collected will have possibly been spun by black widow spiders, proving the concept that DNA from a web collected in the environment can be used to identify the species of spider that spun the web.

A cost‐efficient and simple protocol to enrich prey DNA from extractions of predatory arthropods for large‐scale gut content analysis by Illumina sequencing

This protocol allows for the parallel analysis of several hundred predators in a single MiSeq run, reducing the cost per specimen to only a few dollars and requiring a simple and minimal work flow, and will enable large scale and ecosystem-wide analyses of niche differentiation and food web structure.

Metagenomic analyses reveal previously unrecognized variation in the diets of sympatric Old World monkey species

Results indicate that while overlap exists in the arthropod portion of their diets, 20–25% of taxa consumed are unique to each group, and suggest that variation in arthropid intake may help decrease dietary niche overlap and hence facilitate coexistence of closely-related primate species.

Molecular gut content analysis of different spider body parts

Although all parts of the spider body are suitable for gut-content analysis when using prey-specific PCR approach, results based on metabarcoding suggested the opisthosoma is optimal for detection of predation in spiders because it contained the highest concentration of prey DNA for longer post feeding periods.

Detection of herbivory: eDNA detection from feeding marks on leaves

Many techniques have been developed to investigate the interactions between plants and herbivorous insects in natural environments and are generally used to determine either (a) which plant species

The ecology of environmental DNA and implications for conservation genetics

This work outlines a framework for understanding the ecology of eDNA, including the origin, state, transport, and fate of extraorganismal genetic material, and identifies frontiers of conservation-focused eDNA application where it sees the most potential for growth.

References

SHOWING 1-10 OF 72 REFERENCES

Sparing spiders: faeces as a non-invasive source of DNA

The ability to amplify DNA from spider faeces with specific and general primers suggests that this sample type can be used for diagnostic PCR and sequence-based species and prey identification such as DNA barcoding and next generation sequencing, respectively.

Collembola as alternative prey sustaining spiders in arable ecosystems: prey detection within predators using molecular markers

It is reported the first use of DNA‐based techniques to detect predation by arthropods on natural populations of prey in the field and it was possible to demonstrate that the spiders were exercising prey choice.

Optimizing methods for PCR-based analysis of predation

It is demonstrated that assay sensitivity, in combination with other methodological factors, plays a crucial role to obtain robust trophic interaction data and future work employing molecular prey detection should consider and minimize the methodologically induced variation that would also allow for better cross‐study comparisons.

Identifying spiders through DNA barcodes

This study demonstrates that sequence diversity in a standard segment of the mitochondrial gene coding for cytochrome c oxidase I (COI) is highly effective in discriminating spider species and establishes the potential of COI as a rapid and accurate identification tool for biodiversity surveys of spiders.

Detection of secondary predation by PCR analyses of the gut contents of invertebrate generalist predators

The extreme sensitivity of PCR makes detection of secondary predation more likely, and the only reliable answer in future may be to use PCR to identify, in parallel, instances of intraguild predation.

Carrion fly-derived DNA as a tool for comprehensive and cost-effective assessment of mammalian biodiversity.

It is concluded that the analysis of fly-derived DNA can be implemented in a very rapid and cost-effective manner and will give a relatively unbiased picture of local mammal diversity.

Identifying key cereal aphid predators by molecular gut analysis

PCR is superior to monoclonal antibody technology, giving comparable detectability half‐lives with lower expense, much shorter development times, and greater certainty of a successful outcome, for species‐specific predator gut analysis.

Current progress in DNA barcoding and future implications for entomology

An overview of DNA barcoding and its application to entomology is presented and current advances and future implications of this promising technique are introduced.

DNA sequence from butterfly frass and exuviae

Two species of butterfly were investigated in this study: Vanessa cardui L., the “Painted Lady”, and Pieris rapae L, the ‘Cabbage Butterfly’, and their shed skins (exuviae) may be viewed as another reservoir of DNA.

Herbivory in Spiders: The Importance of Pollen for Orb-Weavers

It is found that about 25% of juvenile orb-weaving spiders’ diet consisted of pollen, the other 75% of flying insects, mainly small dipterans and hymenopterans, suggests that these spiders need to be classified as omnivores rather than pure carnivores.
...