Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells

@article{Lazaris2002SpiderSF,
  title={Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells},
  author={Anthoula Lazaris and Steven Arcidiacono and Yue-Jin Huang and Jiang Feng Zhou and Francois Duguay and Nathalie Chr{\'e}tien and Elizabeth A. Welsh and Jason W. Soares and Costas N. Karatzas},
  journal={Science},
  year={2002},
  volume={295},
  pages={472 - 476}
}
Spider silks are protein-based “biopolymer” filaments or threads secreted by specialized epithelial cells as concentrated soluble precursors of highly repetitive primary sequences. Spider dragline silk is a flexible, lightweight fiber of extraordinary strength and toughness comparable to that of synthetic high-performance fibers. We sought to “biomimic” the process of spider silk production by expressing in mammalian cells the dragline silk genes (ADF-3/MaSpII and MaSpI) of two spider species… 
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High‐toughness Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells
TLDR
This chapter discusses the production of rc-Spider Silk Proteins in the Milk of Transgenic Animals, and the development of transgenic mice for the Spider Dragline Silk Genes.
Recombinant Minimalist Spider Wrapping Silk Proteins Capable of Native-Like Fiber Formation
TLDR
Structural features indicating an assembly of the proteins into spherical structures, fibrils, and silk-like fibers were revealed and CD and Raman spectral analysis of protein secondary structures suggested a transition from predominantly α-helical in solution to increasingly β-sheet in fibers.
Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber
TLDR
The results provide insight into evolution of silk protein size related to mechanical performance, and clarify why spinning lower molecular weight proteins does not recapitulate the properties of native fibers.
Artificial Spider Silk-Recombinant Production and Determinants for Fiber Formation
TLDR
This thesis presents a novel method for the efficient recombinant production of a soluble miniaturized spidroin under non-denaturing conditions, enabling covalent intermolecular cross-linking of proteins constituting the fiber.
Recombinant spider silk from aqueous solutions via a bio-inspired microfluidic chip
TLDR
Artificial spider silks were spun via microfluidic wet-spinning, using a continuous post-spin drawing process (WS-PSD), which partially mimics the spinning process of natural spider silk and substantially contributes to the compact aggregation of microfibrils.
Engineered Large Spider Eggcase Silk Protein for Strong Artificial Fibers
TLDR
In this paper, a large silk-like protein, similar to the repetitive region of a dragline silk protein in size but different in sequence and composition, was produced in high yield in metabolically engineered Escherichia coli.
A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning
TLDR
This protocol, routinely used to spin single micrometer-size fibers from several recombinant silk-like proteins from different spider species, is a powerful tool to generate protein libraries with corresponding fibers for structure–function relationship investigations in protein-based biomaterials.
Production of Synthetic Spider Silk
TLDR
An attempt is made to express synthetic spider silk minifibroins heterologously in Escherichia coli, to purify the recombinant spidroins from cell lysate, and to spin them into artificial fibers through a biomimetic process.
Recombinant Silk Fiber Properties Correlate to Prefibrillar Self-Assembly.
TLDR
Tailoring of dope-state spidroin nanoparticle assembly, thus, appears a promising strategy to modulate fibrillar silk properties.
Spider silk: from soluble protein to extraordinary fiber.
TLDR
This work presents natural and artificial silk production processes, from gene transcription to silk protein processing and finally fiber assembly, which will enable applications of these fascinating protein-based materials in technical and medical sciences.
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