Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells

  title={Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells},
  author={Anthoula Lazaris and Steven Arcidiacono and Yue-Jin Huang and Jiang Feng Zhou and Francois Duguay and Nathalie Chr{\'e}tien and Elizabeth A. Welsh and Jason W. Soares and Costas N. Karatzas},
  pages={472 - 476}
Spider silks are protein-based “biopolymer” filaments or threads secreted by specialized epithelial cells as concentrated soluble precursors of highly repetitive primary sequences. Spider dragline silk is a flexible, lightweight fiber of extraordinary strength and toughness comparable to that of synthetic high-performance fibers. We sought to “biomimic” the process of spider silk production by expressing in mammalian cells the dragline silk genes (ADF-3/MaSpII and MaSpI) of two spider species… 

High‐toughness Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells

This chapter discusses the production of rc-Spider Silk Proteins in the Milk of Transgenic Animals, and the development of transgenic mice for the Spider Dragline Silk Genes.

Recombinant Minimalist Spider Wrapping Silk Proteins Capable of Native-Like Fiber Formation

Structural features indicating an assembly of the proteins into spherical structures, fibrils, and silk-like fibers were revealed and CD and Raman spectral analysis of protein secondary structures suggested a transition from predominantly α-helical in solution to increasingly β-sheet in fibers.

Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber

The results provide insight into evolution of silk protein size related to mechanical performance, and clarify why spinning lower molecular weight proteins does not recapitulate the properties of native fibers.

Artificial Spider Silk-Recombinant Production and Determinants for Fiber Formation

This thesis presents a novel method for the efficient recombinant production of a soluble miniaturized spidroin under non-denaturing conditions, enabling covalent intermolecular cross-linking of proteins constituting the fiber.

Recombinant spider silk from aqueous solutions via a bio-inspired microfluidic chip

Artificial spider silks were spun via microfluidic wet-spinning, using a continuous post-spin drawing process (WS-PSD), which partially mimics the spinning process of natural spider silk and substantially contributes to the compact aggregation of microfibrils.

Engineered Large Spider Eggcase Silk Protein for Strong Artificial Fibers

In this paper, a large silk-like protein, similar to the repetitive region of a dragline silk protein in size but different in sequence and composition, was produced in high yield in metabolically engineered Escherichia coli.

A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning

This protocol, routinely used to spin single micrometer-size fibers from several recombinant silk-like proteins from different spider species, is a powerful tool to generate protein libraries with corresponding fibers for structure–function relationship investigations in protein-based biomaterials.

Production of Synthetic Spider Silk

An attempt is made to express synthetic spider silk minifibroins heterologously in Escherichia coli, to purify the recombinant spidroins from cell lysate, and to spin them into artificial fibers through a biomimetic process.

Recombinant Silk Fiber Properties Correlate to Prefibrillar Self-Assembly.

Tailoring of dope-state spidroin nanoparticle assembly, thus, appears a promising strategy to modulate fibrillar silk properties.

Spider silk: from soluble protein to extraordinary fiber.

This work presents natural and artificial silk production processes, from gene transcription to silk protein processing and finally fiber assembly, which will enable applications of these fascinating protein-based materials in technical and medical sciences.



Microbial production of spider silk proteins.

Production of spider silk proteins in tobacco and potato

The accumulation of recombinant silk proteins, which are encoded by synthetic genes of 420–3,600 base pairs, up to a level of at least 2% of total soluble protein in the endoplasmic reticulum of tobacco and potato leaves and potato tubers, respectively are demonstrated.

Regenerated Spider Silk: Processing, Properties, and Structure

Spider dragline silk is Nature's high-performance protein fiber. This biomaterial has attracted much interest from scientists in various disciplines since it has become feasible to produce spider

Liquid crystalline spinning of spider silk

Successful copying of the spider's internal processing and precise control over protein folding, combined with knowledge of the gene sequences of its spinning dopes, could permit industrial production of silk-based fibres with unique properties under benign conditions.

The mechanical design of spider silks: from fibroin sequence to mechanical function.

Comparison of MA silks from Araneus diadematus and Nephila clavipes shows variation in fibroin sequence and properties between spider species provides the opportunity to investigate the design of these remarkable biomaterials.

Structure of a protein superfiber: spider dragline silk.

  • M. XuR. Lewis
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1990
A model for the physical properties of fiber formation, strength, and elasticity, based on this repetitive protein sequence of spider major ampullate (dragline) silk, is presented.

A Microfabricated Wet-Spinning Apparatus To Spin Fibers of Silk Proteins. StructureProperty Correlations

A microfabricated spinneret is described that is capable of spinning meters of fibers from solutions containing as little as 10 mg of purified protein. Using the spinneret, regenerated Bombyx mori

Silk Properties Determined by Gland-Specific Expression of a Spider Fibroin Gene Family

A gene family from the spider Araneus diadematus was found to encode silk-forming proteins (fibroins) with different proportions of amorphous glycine-rich domains and crystal domains built from poly(alanine) and poly(glycine-alanine), which allows for a range of mechanical properties according to the crystal-forming potential of the constituent fibroins.

Comparative Structural Characterization of Naturally- and Synthetically-Spun Fibers of Bombyx mori Fibroin

This investigation describes the comparative structural characterization of naturally- and synthetically-spun fibers of Bombyx mori fibroin. Wet spinning from 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP)