Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells

@article{Lazaris2002SpiderSF,
  title={Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells},
  author={Anthoula Lazaris and Steven Arcidiacono and Yue-Jin Huang and Jiang Feng Zhou and Francois Duguay and Nathalie Chr{\'e}tien and Elizabeth A. Welsh and Jason W. Soares and Costas N. Karatzas},
  journal={Science},
  year={2002},
  volume={295},
  pages={472 - 476}
}
Spider silks are protein-based “biopolymer” filaments or threads secreted by specialized epithelial cells as concentrated soluble precursors of highly repetitive primary sequences. Spider dragline silk is a flexible, lightweight fiber of extraordinary strength and toughness comparable to that of synthetic high-performance fibers. We sought to “biomimic” the process of spider silk production by expressing in mammalian cells the dragline silk genes (ADF-3/MaSpII and MaSpI) of two spider species… 
High‐toughness Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells
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This chapter discusses the production of rc-Spider Silk Proteins in the Milk of Transgenic Animals, and the development of transgenic mice for the Spider Dragline Silk Genes.
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This protocol, routinely used to spin single micrometer-size fibers from several recombinant silk-like proteins from different spider species, is a powerful tool to generate protein libraries with corresponding fibers for structure–function relationship investigations in protein-based biomaterials.
Production of Synthetic Spider Silk
TLDR
An attempt is made to express synthetic spider silk minifibroins heterologously in Escherichia coli, to purify the recombinant spidroins from cell lysate, and to spin them into artificial fibers through a biomimetic process.
Recombinant Silk Fiber Properties Correlate to Prefibrillar Self-Assembly.
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Tailoring of dope-state spidroin nanoparticle assembly, thus, appears a promising strategy to modulate fibrillar silk properties.
Spider silk: from soluble protein to extraordinary fiber.
TLDR
This work presents natural and artificial silk production processes, from gene transcription to silk protein processing and finally fiber assembly, which will enable applications of these fascinating protein-based materials in technical and medical sciences.
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