Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate.

  title={Sphingosine 1-phosphate lyase enzyme assay using a BODIPY-labeled substrate.},
  author={Padmavathi Bandhuvula and Zaiguo Li and Robert Bittman and Julie D Saba},
  journal={Biochemical and biophysical research communications},
  volume={380 2},
Sphingosine 1-phosphate lyase (SPL) is responsible for the irreversible catabolism of sphingosine 1-phosphate, which signals through five membrane receptors to mediate cell stress responses, angiogenesis, and lymphocyte trafficking. The standard assay for SPL activity utilizes a radioactive dihydrosphingosine 1-phosphate substrate and is expensive and cumbersome. In this study, we describe an SPL assay that employs an omega-labeled BODIPY-sphingosine 1-phosphate substrate, allowing fluorescent… CONTINUE READING
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