Spheroid culture for enhanced differentiation of human embryonic stem cells to hepatocyte-like cells.

@article{Subramanian2014SpheroidCF,
  title={Spheroid culture for enhanced differentiation of human embryonic stem cells to hepatocyte-like cells.},
  author={K. N. Subramanian and Derek Jason Owens and Ravali Raju and Meri T. Firpo and Timothy O'Brien and Catherine M. Verfaillie and Wei-Shou Hu},
  journal={Stem cells and development},
  year={2014},
  volume={23 2},
  pages={124-31}
}
Stem cell-derived hepatocyte-like cells hold great potential for the treatment of liver disease and for drug toxicity screening. The success of these applications hinges on the generation of differentiated cells with high liver specific activities. Many protocols have been developed to guide human embryonic stem cells (hESCs) to differentiate to the hepatic lineage. Here we report cultivation of hESCs as three-dimensional aggregates that enhances their differentiation to hepatocyte-like cells… CONTINUE READING
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Current status of hepatocyte

  • RD Hughes
  • TRANSPLANTATION
  • 2012

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