• DOI:10.1055/s-0033-1360302
• Corpus ID: 21239802

Sphaeralcic acid and tomentin, anti-inflammatory compounds produced in cell suspension cultures of Sphaeralcea angustifolia.

@article{PrezHernndez2014SphaeralcicAA,
  title={Sphaeralcic acid and tomentin, anti-inflammatory compounds produced in cell suspension cultures of Sphaeralcea angustifolia.},
  author={Juan Bernardo P{\'e}rez-Hern{\'a}ndez and Manas{\'e}s Gonz{\'a}lez-Cortazar and Silvia Marquina and Maribel Herrera-Ruiz and Mariana Meckes-Fischer and Jaime Tortoriello and Francisco Cruz-Sosa and Mar{\'i}a del Pilar Nicasio-Torres},
  journal={Planta medica},
  year={2014},
  volume={80 2-3},
  pages={
          209-14
        }
}

• J. Pérez-Hernández, M. González-Cortazar, +5 authors M. Nicasio-Torres
• Published 31 January 2014
• Chemistry, Biology
• Planta medica

Sphaeralcea angustifolia, an endangered plant species in Mexico, is employed to treat inflammatory processes and as a wound healing remedy. Scopoletin (1) was reported as one of the main bioactive compounds in this plant. Here, we isolated and identified compounds with anti-inflammatory properties from the suspension-cultured cells of S. angustifolia. The CH2Cl2 : CH3OH extract of the cells exhibited anti-inflammatory properties in acute inflammation models. Two compounds were isolated, 5-hydroxy-6,7-dimethoxycoumarin, named tomentin (2), and 2-(1,8-dihydroxy-4-isopropyl-6-methyl-7-methoxy)-naphthoic acid, denominated as sphaeralcic acid (3). Their structures were determined by spectroscopic and spectrometric analyses. The anti-inflammatory effects of both compounds were also evaluated. At a dose of 45 mg/kg, compound 2 inhibited the formation of λ-carrageenan footpad edema at 58 %, and compound 3 at 66 %. Local application of compound 2 (225 mM per ear) or 3 (174 mM per ear) inhibited the phorbol ester-induced auricular edema formation by 57 % or 86 %, respectively. The effect of compound 3 was dose-dependent and the ED50 was 93 mM.